购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

Palomid 529

产品编号 T2706Cas号 914913-88-5
别名 P529, SG 00529

Palomid 529 (SG 00529) 是一种mTORC1和mTORC2复合体抑制剂,已用于研究治疗年龄相关性黄斑变性的试验。

Palomid 529
TargetMol

为众多的药物研发团队赋能,

让新药发现更简单!

Palomid 529

纯度: 99.03%
产品编号 T2706 别名 P529, SG 00529Cas号 914913-88-5

Palomid 529 (SG 00529) 是一种mTORC1和mTORC2复合体抑制剂,已用于研究治疗年龄相关性黄斑变性的试验。

规格价格库存数量
1 mg¥ 186现货
5 mg¥ 413现货
10 mg¥ 662现货
25 mg¥ 1,260现货
50 mg¥ 1,980现货
100 mg¥ 2,970现货
1 mL x 10 mM (in DMSO)¥ 455现货
大包装 & 定制
加入购物车
实验操作小课堂
查看更多

"Palomid 529"的相关化合物库

选择批次:
纯度:99.03%
联系我们获取更多批次信息
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。

产品介绍

生物活性
产品描述
Palomid 529 (SG 00529) has been used in trials studying the treatment of Age-Related Macular Degeneration.
体外活性
Palomid 529抑制肿瘤生长,血管生成和血管通透性.在兔视网膜脱离模型中,Palomid 529可有效抑制Müller细胞增殖,神经胶质瘢痕形成和感光细胞死亡.Palomid 529剂量依赖性抑制Ad-VEGF-A驱动的血管生成,裸鼠灌胃Palomid 529可抑制C6V10胶质瘤生长.与对照组相比,Palomid 529处理PC-3肿瘤小鼠可减少57.1%的肿瘤生长.Palomid 529明显抑制小鼠Brca1缺失肿瘤生长模型中Akt和 mTOR信号通路.
体内活性
Palomid529不仅降低缺血性视网膜的增殖,而且能改善血管形成的组织和结构。Palomid 529抑制VEGF介导和bFGF介导的内皮细胞增殖,IC 50分别为20 nM 和30 nM。Palomid 529可诱导内皮细胞凋亡,降低血管内皮生长因子VEGF-A诱导的pAktS473,pGSK3βS9和pS6磷酸化。在肺癌NCI- 60细胞系中,Palomid 529显示高效的抗增殖活性,GI50< 35 μM。此外,Palomid 529可显著增强辐射对前列腺癌细胞(PC-3)的抗增殖作用。Palomid 529对PC-3细胞产生浓度依赖性生长抑制。Palomid 529抑制PC-3中辐射诱导的p-Akt激活,并降低Bcl-2/Bax的比例。
激酶实验
Estrogen receptor binding assays: The proteins are produced with rabbit reticulocyte lysates. The amount of template used in each reaction is determined empirically and expression is monitored in parallel reactions where [35S]methionine is incorporated into the receptor followed by gel electrophoresis and exposure to film. Binding reactions of the estrogen receptors (ER) and Palomid 529 are carried out in 100 mL final volumes in TEG buffer [10 mM Tris (pH 7.5), 1.5 mM EDTA, 10% glycerol]. In vitro transcribed-translated receptor (5 μL) is used in each binding reaction in the presence of 0.5 nM [3H]estradiol (E2). Palomid 529 is routinely tested from 10?11 to 10?6 M and diluted in ethanol. The reactions are incubated at 4 °C overnight and bound E2 is quantified by adding 200 mL dextran-coated charcoal. After a 15-minutes rotation at 4 °C, the tubes are centrifuged for 10 minutes and 150 mL of the supernatant are added to 5 mL scintillation mixture for determination of cpm by liquid scintillation counting. The maximum binding is determined by competing bound E2 with only the ethanol vehicle. Controls for background are included in each experiment using 5 mL unprogrammed rabbit reticulocyte lysate. This value, typically 10% to 15% of the maximal counts, is subtracted from all values. The data are plotted and Ki values are calculated. Experiments are conducted at least thrice in duplicate.
细胞实验
Human umbilical vascular endothelial cells (HUVEC) are used. The proliferation assay is carried out by seeding the HUVECs in 96-well plates at a density of 1,000 per well in complete medium. Following a 24-hour plating period, the cells are starved for 24 hours in 0.5% serum before being treated with Palomid 529 in the presence of 10 ng/mL basic fibroblast growth factor (bFGF) or VEGF in complete medium. After 48 hours, cell number is determined using a colorimetric method. The results are expressed as the percentage of the maximal bFGF or VEGF response in the absence of Palomid 529. Nonproliferating endothelial cells are assayed by growing HUVECs to quiescence in 96-well plates and treating with Palomid 529 for 48 hours. Initially, 5,000 cells per well are seeded and confluence is achieved the next day. The plates are incubated for another 24 hours to ensure growth arrest before treatment with Palomid 529. (Only for Reference)
别名P529, SG 00529
化学信息
分子量406.43
分子式C24H22O6
CAS No.914913-88-5
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 50 mg/mL (123.02 mM), Sonication is recommended.
溶液配制表
DMSO
1mg5mg10mg50mg
1 mM2.4604 mL12.3022 mL24.6045 mL123.0224 mL
5 mM0.4921 mL2.4604 mL4.9209 mL24.6045 mL
10 mM0.2460 mL1.2302 mL2.4604 mL12.3022 mL
20 mM0.1230 mL0.6151 mL1.2302 mL6.1511 mL
50 mM0.0492 mL0.2460 mL0.4921 mL2.4604 mL
100 mM0.0246 mL0.1230 mL0.2460 mL1.2302 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

对于不同动物的给药剂量换算,您也可以参考 更多

关键词

Related Tags: buy Palomid 529 | purchase Palomid 529 | Palomid 529 cost | order Palomid 529 | Palomid 529 chemical structure | Palomid 529 in vivo | Palomid 529 in vitro | Palomid 529 formula | Palomid 529 molecular weight